5.14- Humulin

Understand that large amounts of human insulin can be manufactured from genetically modified bacteria that are grown in a fermenter

• A culture of the bacteria will be injected into the fermenter
• Nutrients need to be provided 
• Gases, pH and temperature need to controlled
• By creating the optimal temp. --> population will increase and the bacteria will manufacture the protein insulin
• The bacteria inside the fermenter will manufacture the insulin protein from amino acids
• Then the product needs to be removed and purification needs to be carried out (downstream processing)
• Genetically engineered human insulin is called Humulin